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goat anti human spp1 antibody  (R&D Systems)


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    R&D Systems goat anti human spp1 antibody
    ( A–D, F–G ) UMAP (as in ) illustration of the normalized expression of selected ligand and receptor gene pairs. Red dashed lines in figures A, B and D mark the erythroid clusters. The blow-up shown in ( G ) is to better visualize PDGFA- and PDGFB-expressing cells. ( E ) Left panel: Formalin-fixed, paraffin-embedded (FFPE) human BM slides were sequentially stained for DAPI (blue), CD45 (yellow), <t>SPP1</t> (red), CD271 (pink), and NCAM1 (cyan) and scanned with an OlympusVS120 slide scanner. Left upper image: all markers are shown; left lower image: just NCAM1 channel is shown. Middle panel: confocal laser scanning analysis of BM biopsies co-stained with CD271 (red), NCAM1 (green), SPP1 (white), and DAPI (blue) presented as 3D orthographic cross-section view. Right panel: Single staining channel data for CD271 (red), NCAM1 (green), SPP1 (white), and corresponding blow-ups for indicated areas. NCAM1, SPP1 and CD271(NGFR). White scale bars represent 50 μm and yellow scale bars represent 25 μm. White dashed lines indicate the trabecular bone surface lining regions.
    Goat Anti Human Spp1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+spp1+antibody/pmc10097421-45-41-45?v=R%26D+Systems
    Average 93 stars, based on 91 article reviews
    goat anti human spp1 antibody - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Identification of phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow based on single-cell RNA sequencing"

    Article Title: Identification of phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow based on single-cell RNA sequencing

    Journal: eLife

    doi: 10.7554/eLife.81656

    ( A–D, F–G ) UMAP (as in ) illustration of the normalized expression of selected ligand and receptor gene pairs. Red dashed lines in figures A, B and D mark the erythroid clusters. The blow-up shown in ( G ) is to better visualize PDGFA- and PDGFB-expressing cells. ( E ) Left panel: Formalin-fixed, paraffin-embedded (FFPE) human BM slides were sequentially stained for DAPI (blue), CD45 (yellow), SPP1 (red), CD271 (pink), and NCAM1 (cyan) and scanned with an OlympusVS120 slide scanner. Left upper image: all markers are shown; left lower image: just NCAM1 channel is shown. Middle panel: confocal laser scanning analysis of BM biopsies co-stained with CD271 (red), NCAM1 (green), SPP1 (white), and DAPI (blue) presented as 3D orthographic cross-section view. Right panel: Single staining channel data for CD271 (red), NCAM1 (green), SPP1 (white), and corresponding blow-ups for indicated areas. NCAM1, SPP1 and CD271(NGFR). White scale bars represent 50 μm and yellow scale bars represent 25 μm. White dashed lines indicate the trabecular bone surface lining regions.
    Figure Legend Snippet: ( A–D, F–G ) UMAP (as in ) illustration of the normalized expression of selected ligand and receptor gene pairs. Red dashed lines in figures A, B and D mark the erythroid clusters. The blow-up shown in ( G ) is to better visualize PDGFA- and PDGFB-expressing cells. ( E ) Left panel: Formalin-fixed, paraffin-embedded (FFPE) human BM slides were sequentially stained for DAPI (blue), CD45 (yellow), SPP1 (red), CD271 (pink), and NCAM1 (cyan) and scanned with an OlympusVS120 slide scanner. Left upper image: all markers are shown; left lower image: just NCAM1 channel is shown. Middle panel: confocal laser scanning analysis of BM biopsies co-stained with CD271 (red), NCAM1 (green), SPP1 (white), and DAPI (blue) presented as 3D orthographic cross-section view. Right panel: Single staining channel data for CD271 (red), NCAM1 (green), SPP1 (white), and corresponding blow-ups for indicated areas. NCAM1, SPP1 and CD271(NGFR). White scale bars represent 50 μm and yellow scale bars represent 25 μm. White dashed lines indicate the trabecular bone surface lining regions.

    Techniques Used: Expressing, Formalin-fixed Paraffin-Embedded, Staining

    ( A–E, G–H, J ) UMAP (as in ) illustration of the normalized expression of selected ligand and receptor gene pairs. ( F ) Flow cytometric analysis of SPP1 expression of primary CD45 low/- CD235a - CD71 - CD271 + CD56 + cells (red histogram), CD45 low/- CD235a - CD71 - CD271 + CD56 - CD81 ++ cells (blue histogram) and corresponding isotype control (orange histogram). ( I ) CFU-F assay of sorted CD45 low/- CD235a - CD71 - CD271 + cells (100 cells/well) in the presence (upper panel) or absence (lower panel) of sorted bone marrow CD45 + cells (3x10 5 cells/well), respectively.
    Figure Legend Snippet: ( A–E, G–H, J ) UMAP (as in ) illustration of the normalized expression of selected ligand and receptor gene pairs. ( F ) Flow cytometric analysis of SPP1 expression of primary CD45 low/- CD235a - CD71 - CD271 + CD56 + cells (red histogram), CD45 low/- CD235a - CD71 - CD271 + CD56 - CD81 ++ cells (blue histogram) and corresponding isotype control (orange histogram). ( I ) CFU-F assay of sorted CD45 low/- CD235a - CD71 - CD271 + cells (100 cells/well) in the presence (upper panel) or absence (lower panel) of sorted bone marrow CD45 + cells (3x10 5 cells/well), respectively.

    Techniques Used: Expressing, Control



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    Image Search Results


    ( A–D, F–G ) UMAP (as in ) illustration of the normalized expression of selected ligand and receptor gene pairs. Red dashed lines in figures A, B and D mark the erythroid clusters. The blow-up shown in ( G ) is to better visualize PDGFA- and PDGFB-expressing cells. ( E ) Left panel: Formalin-fixed, paraffin-embedded (FFPE) human BM slides were sequentially stained for DAPI (blue), CD45 (yellow), SPP1 (red), CD271 (pink), and NCAM1 (cyan) and scanned with an OlympusVS120 slide scanner. Left upper image: all markers are shown; left lower image: just NCAM1 channel is shown. Middle panel: confocal laser scanning analysis of BM biopsies co-stained with CD271 (red), NCAM1 (green), SPP1 (white), and DAPI (blue) presented as 3D orthographic cross-section view. Right panel: Single staining channel data for CD271 (red), NCAM1 (green), SPP1 (white), and corresponding blow-ups for indicated areas. NCAM1, SPP1 and CD271(NGFR). White scale bars represent 50 μm and yellow scale bars represent 25 μm. White dashed lines indicate the trabecular bone surface lining regions.

    Journal: eLife

    Article Title: Identification of phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow based on single-cell RNA sequencing

    doi: 10.7554/eLife.81656

    Figure Lengend Snippet: ( A–D, F–G ) UMAP (as in ) illustration of the normalized expression of selected ligand and receptor gene pairs. Red dashed lines in figures A, B and D mark the erythroid clusters. The blow-up shown in ( G ) is to better visualize PDGFA- and PDGFB-expressing cells. ( E ) Left panel: Formalin-fixed, paraffin-embedded (FFPE) human BM slides were sequentially stained for DAPI (blue), CD45 (yellow), SPP1 (red), CD271 (pink), and NCAM1 (cyan) and scanned with an OlympusVS120 slide scanner. Left upper image: all markers are shown; left lower image: just NCAM1 channel is shown. Middle panel: confocal laser scanning analysis of BM biopsies co-stained with CD271 (red), NCAM1 (green), SPP1 (white), and DAPI (blue) presented as 3D orthographic cross-section view. Right panel: Single staining channel data for CD271 (red), NCAM1 (green), SPP1 (white), and corresponding blow-ups for indicated areas. NCAM1, SPP1 and CD271(NGFR). White scale bars represent 50 μm and yellow scale bars represent 25 μm. White dashed lines indicate the trabecular bone surface lining regions.

    Article Snippet: For SPP1 staining, after deparaffinization, rehydration, and antigen retrieval, slides were permeabilized with 0.1% Triton X100 (Sigma Ultra, T9284) solution at 37 °C for 15 min, blocked using donkey serum (Jackson Immuno Research, 017-000-121), and stained overnight at 4 °C with goat anti-human SPP1 antibody (R&D Systems, AF1433-SP), followed by secondary staining with donkey anti-goat AF594 (Jackson ImmunoResearch, 706-585-147) and staining with CD56 (NCAM1)-AF647 plus DAPI.

    Techniques: Expressing, Formalin-fixed Paraffin-Embedded, Staining

    ( A–E, G–H, J ) UMAP (as in ) illustration of the normalized expression of selected ligand and receptor gene pairs. ( F ) Flow cytometric analysis of SPP1 expression of primary CD45 low/- CD235a - CD71 - CD271 + CD56 + cells (red histogram), CD45 low/- CD235a - CD71 - CD271 + CD56 - CD81 ++ cells (blue histogram) and corresponding isotype control (orange histogram). ( I ) CFU-F assay of sorted CD45 low/- CD235a - CD71 - CD271 + cells (100 cells/well) in the presence (upper panel) or absence (lower panel) of sorted bone marrow CD45 + cells (3x10 5 cells/well), respectively.

    Journal: eLife

    Article Title: Identification of phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow based on single-cell RNA sequencing

    doi: 10.7554/eLife.81656

    Figure Lengend Snippet: ( A–E, G–H, J ) UMAP (as in ) illustration of the normalized expression of selected ligand and receptor gene pairs. ( F ) Flow cytometric analysis of SPP1 expression of primary CD45 low/- CD235a - CD71 - CD271 + CD56 + cells (red histogram), CD45 low/- CD235a - CD71 - CD271 + CD56 - CD81 ++ cells (blue histogram) and corresponding isotype control (orange histogram). ( I ) CFU-F assay of sorted CD45 low/- CD235a - CD71 - CD271 + cells (100 cells/well) in the presence (upper panel) or absence (lower panel) of sorted bone marrow CD45 + cells (3x10 5 cells/well), respectively.

    Article Snippet: For SPP1 staining, after deparaffinization, rehydration, and antigen retrieval, slides were permeabilized with 0.1% Triton X100 (Sigma Ultra, T9284) solution at 37 °C for 15 min, blocked using donkey serum (Jackson Immuno Research, 017-000-121), and stained overnight at 4 °C with goat anti-human SPP1 antibody (R&D Systems, AF1433-SP), followed by secondary staining with donkey anti-goat AF594 (Jackson ImmunoResearch, 706-585-147) and staining with CD56 (NCAM1)-AF647 plus DAPI.

    Techniques: Expressing, Control